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KMID : 0665220120250030570
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Korean Journal of Food and Nutrition
2012 Volume.25 No. 3 p.570 ~ p.578
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Optimization of Compound K Production from Ginseng Extract by Enzymatic Bioconversion of Trichoderma reesei
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Han Gang
Lee Nam-Keun
Lee Yu-Ri
Jeong Eun-Jeong
Jeong Yong-Seob
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Abstract
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Compound K(ginsenoside M1) is one of saponin metabolites and has many benefits for human health. This study was to investigate Compound K produced from ginseng crude saponin extract with commercial cellulolytic complex enzyme(cellulase, β-glucanase, and hemicellulase) obtained from Trichoderma reesei. The effect factors(temperature, pH, ginseng crude saponin extract and enzyme concentration, and reaction time) on production of Compound K from ginseng crude saponin extract were determined by one factor at a time method. The selected major factor variables were ginseng crude saponin extract of 2%(w/v), enzyme of 7%(v/v), reaction time of 48 hr. Based on the effect factors, response surface method was proceeded to optimize the enzymatic bioconversion conditions for the desirable Compound K production under the fixed condition of pH 5.0 and 50℃. The optimal reaction condition from RSM was ginseng crude saponin extract of 2.38%, enzyme of 6.06%, and reaction time of 64.04 hr. The expected concentration of Compound K produced from that reaction was 840.77 ㎎/100 g. Production of Compound K was 1, 017.93 ㎎/100 g and 862.31 ㎎/100 g, by flask and bench-scale bioreactor(2.5ℓ) system, respectively.
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KEYWORD
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Compound K, ginseng saponin, response surface methodology, Trichoderma reesei, enzymatic bioconversion
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